Single cell 3' RNA sequencing of WM239A melanoma cell line and its highly metastatic subclones

The molecular mechanism of melanoma metastasis is poorly understood. The aim of our study was to delineate expression kinetics during the acquisition of metastatic potential, and thereby to characterize subclone of primary cell line responsible for metastasis. We performed single-cell RNA sequencing of poorly metastatic melanoma cell line (WM239A), its subclones with high potential of lung (113/6-4L) and brain metastasis (131/4-5B1 and 131/4-5B2). Single-cell RNA sequencing libraries were generated using Chromium Single-cell 3' Reagent Kits v2 (10x Genomics, Pleasanton, CA) according to the manufacturer’s protocol.