Shotgun proteomics of Roseburia grown on human milk oligosaccharides and of Roseburia grown on mucin in co-culture with Akkermansia muciniphilia.

The main objective of the present proteomic study is to identify the metabolic response, in particular theglycan uptake and degradation machinery, conferring members of Roseburia growth on HMOs and/or onrelated O-glycans. Accordingly, the proteomes of R. hominis and R. inulinivorans bothgrown on humanmilk oligosaccharides (HMOs), were compared to glucose to reveal the molecular basis for growth onHMOs. Furthermore, we compare the proteomes of R. hominis and R. inulinivorans grownin co-culturewith A. muciniphilia either on mucin or on glucose to identify potential metabolic routes of mucin derivedO-glycan utilization in Roseburia.