Resistance gene insertion sites in IncHI1 plasmids inferred from a combination of PCR and sequencing.

Summaries of five insertion patterns are shown in bold italics; these are inferred from sequence data where available (italics) and PCR using primers shown in Table 3 (labelled G–Q). STy = Salmonella enterica serovar Typhi, SCh = Salmonella enterica serovar Choleraesuis, STm = Salmonella enterica serovar Typhimurium, SPa = Salmonella enterica serovar Paratyphi A, Ec = E. coli O111:H−. + positive PCR result (i.e. successful amplification); - negative PCR result (i.e. no amplification product detected);*distinct amplicon size for PST1; n/d PCR not done; n/a sequence data not available. “strAB 2nd” copy refers to the insertion of streptomycin resistance genes strAB directly into the plasmid backbone (SPAP0230-SPAP0231), not as part of the bla/sul/str element (SPAP0152-SPAP0153).