Plasmidsa.

aAll plasmids were prepared in this laboratory.bDescribed in [11], some illustrated in Fig. 1A.cDescribed in [37], [69], illustrated in Figures 1, S3.dDeletes 60 bp within O between 38759 and 38820, include one of two BglII sites.e∼1450 bp BglII DNA fragment with KanR (derived from Tn903) within pUC4K was inserted within the remaining BglII site in p51.fRemoval of ∼1426 bp PstI fragment from ∼1450 bp KanR fragment, adding 24 bp within the 60 bp deletion region between bases 38759 and 38820.gInitially 45 random bases were chosen, but then some bases were modified to remove the possibility for secondary structure (hairpin) formation.hExpression plasmid [74]–[76] where O expression is regulated by CI[Ts]857 repressor from pR promoter.iPart of cI857 –pR in pcIpR-O-timm was replaced with 379 bp N-terminal 434-cI[Ts]–pR sequence, resulting in constitutive expression of O[75].