Role of YAP in Liver Regeneration After Acetaminophen Overdose

Overdose of acetaminophen (APAP) is the major cause of acute liver failure in the Western world with very limited treatment options. Previous studies from our groups and others have shown that timely activation of liver regeneration is a critical determinant of transplant-free survival of APAP-induced acute liver failure (ALF) patients. We used affy microarrays to explore the mechanisms of transcriptional expression in YAP-KO mice after 300mg/kg APAP overdose. Livers were extracted from wildtype and YAP-KO male mice that were fasted for 0 hour time points. Intraperitoneal injection of 300 mg/kg acetaminophen overdose was given to fasted mice and livers were extracted 24 hours after overdose. RNA was extracted from 100 mg of liver tissue and pooled together (n of 3) to be used for microarray analysis.