Single-Turnover DNA Cleavage Rate Constants (kobs) using 1 μM E301W SgrAI and 1 nM 32P-labeled 40 bp primary site (40–1) DNA.

aValues presented at the average of three independent trials ± the standard deviation.bPercent Cleaved is the percentage of DNA cleaved by SgrAI in the assay by the given rate constant (kobs). In cases where less than 100% is cleaved by the given rate constant, the remainder is cleaved by a nonaccelerated rate constant.cRelative Acceleration is the ratio of kobs in the presence of 1000 μM PC DNA to that in the absence of added PC DNA.Single-Turnover DNA Cleavage Rate Constants (kobs) using 1 μM E301W SgrAI and 1 nM 32P-labeled 40 bp primary site (40–1) DNA.