Sexes of Rhabditis sp. SB347 F1 from selfing or outcrossing parents.

Data from Figure 2a. To determine the sex of the F1 from selfing hermaphrodites, the self-progeny of 20 SB347 animals was collected throughout their lifespan. The hermaphrodite mothers were selected by first isolating dauers, which in SB347 always develop into hermaphrodites. Dauers were placed into single 6 cm agar plates seeded with E. coli OP50-1 and left to mature into adults. Every 8 h of their reproductive life, the hermaphrodite mothers were transferred to a new plate until they stopped laying eggs. To control more precisely for worms that may escape the plate before being scored, we sexed the progeny while they were at larval stages (two days after hatching). We scored the three sexes using several traits, including developmental rates and morphologies. Dauer larvae (destined to become hermaphrodites) are thin with darkly-pigmented guts. Larger larvae with long thin tails develop into females. Male larvae have a characteristically blunt tail.