For clinical deep sequencing process, construct the process with small amount input gDNA( ~ 50ng) and validate. To test, only human tumor cell lines were used.
To reveal the functional characteristics and regulating mechanism of the GTF2I-RARA fusion gene, we established a GTF2I-RARA-transfected HL60 cell model and exam its sensitivity to ATRA by western-blo
There are 5 gDNA standard samples (including at least 2 bio-replicate data and diluted 3,6,12 times replicate data) to validate the feasibility of the SEGF method.