In vivo identification of Toxoplasma gondii antigenic proteins and in silico study of their polymorphism.

This study aims to identify novel candidates for the development of serotyping assays for Toxoplasma gondii (T. gondii). We employed an in vivo approach, utilizing co-immunoprecipitation (Co-IP) with sera from infected mice to identify antigenic proteins, followed by detection via LC-MS/MS. Additionally, an in silico analysis was conducted to explore the genetic polymorphism of the genes encoding these proteins and to determine mutations associated with specific genotypes.