Identification of the EHV-1 immediate-early gene through the inhibition of protein synthesis.

In this experiment, we utilized two concentrations of CHX (20 and 100 mg/ml) and observed the effects at two different incubation durations (6 and 8 hours). Our findings unequivocally indicate that ORF64 is the sole immediate early gene of EHV-1. The numbers refer to the number of the detected reads. Several EHV-1 genes exhibit low-level expression, a phenomenon that can be attributed to transcriptional noise, which becomes more pronounced with reduced CHX concentrations and extended exposure times. (XLSX)