Developmental trajectory of unconventional T cells of the cynomolgus macaque thymus

We profiled the differentiation pathways and heterogeneity of thymocytes in the young cynomolgus monkey thymus using single-cell RNA sequencing. Overall design: Using DEG analysis and trajectory analysis, we temporally distinguished the conventional selection pathway and the agonist selection pathway of thymocytes and inferred the association between agonist selection and various unconventional T-cell lineages.