Differential expression of miRNA in stage-specific prostate cancer

Dysregulation of microRNAs (miRNAs) can majorly contribute to prostate cancer tumor development, progression, and are differentially expressed in normal and cancer tissues. Glass-based chip assay was designed for prostate cancer cell lines; from early disease stage to advance and to castration resistance prostate cancer (CRPC). We have used glass based chip microarray assay (miRNA-microarray); and our attempt identified that the expression of miR-301 was upregulated at early stage and miR- 146/146b showed increased in expression during the advanced stage of prostate cancer. While the expression of miR-205 and miR-221 was consistently downregulated across all the disease stage; from early to advanced and also during the CRPC stage. Also, we have identified some disease-specific/signature miRNAs for e.g. miR-204 which was differentially expressed only during early stage of prostate cancer and miR-520 was during CRPC stage. The expression of non coding RNA (miRNA) of normal prostate cell line and prostate cancer cell lines (AR positive and AR negative) were analyzed