Mechanisms of drugs-resistance in small cell lung cancer II

Malignant transformation of SCLC often occurs simultaneously with the acquisition of chemotherapy resistance, suggesting that phenotypic malignant transformation is associated with adaptation to chemotherapy-induced stress. Recently, it has been reported that autophagy deficiency is involved in chemotherapy resistance in SCLC. To analyze gene expression changes associated with SCLC malignant transformation, we established ATG7 knockout and ATG7/SQSTM1 double knockout SCLC cell lines and performed RNA sequencing. ATG7 Knockout and ATG7/SQSTM1 double knockout DMS273 cells were generated by CRISPRv2 vector. RNAs were isolated from each generated DMS273 cells and subjected RNA-sequencing analysis.