Consequences of ZNF292 deficiency for human cortical interneuron development

Human embryonic stem cell (hESC) models of ZNF292 deficiency were generated, including a KRAB-only control and two CRISPRi knockdown models (ZNF-G1 and ZNF-G2). These hESC models were used to derived MGE-like ventral telencephalic neural progenitors (day 15; D15) and cortical interneurons (day 30; D30). RNA-seq was conducted in these models to define transcriptional changes that result from ZNF292 deficiency during this process. Overall design: For each time point, 4 biological replicates generated from independent differentiations of hESCs were used for comparison of transcriptomic differences between control (KRAB) and ZNF292 CRISPRi-mediated deficient (ZNF-G1 and ZNF-G2) progenitors and neurons.