Bacterial rapid adaptation to alkaline environments

To investigate the rapid adaptation mechanism of Bacillus thuringiensis in an alkaline environment, we have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gene expression between normal condition and alkaline condition. Bt HD73 strain was cultured in LB at 30 °C and 220 rpm. NaOH was added at a concentration of 28 mM to the cultures when the OD600 reached 0.7. After 10 minutes of incubation, total RNA was extracted