Comparison of transport activities for different NTT1 fusions and deletion.
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Relative NTT1-WT activities were determined from specific radioactive ATP uptake (using 50 µM ATP) performed during 5 minutes for wild type (WT) NTT1 and for mutants NTT1-K155E, NTT1-K155R, NTT1-E245K, NTT1-K527E and NTT1-K527R. The activity of WT is defined as 100% activity. The VM and KM values for ATP and the KM value for ADP were determined as described in [6] using the radioactive ATP uptake method for ATP and the luminescence approach for ADP. Each measurement is the mean of three independent experiments.
创建时间：
2015-12-02
