Raw diffraction images for oxalate bound murine Fumarylacetoacetate Domain Containing Protein 1 (FAHD1)

FAH domain-containing protein 1 (FAHD1) is a mitochondrial protein displaying both acylpyruvate hydrolase (ApH) and oxaloacetate decarboxylase (ODx) activity. As mitochondrial ODx, FAHD1 acts antagonistically to pyruvate carboxylase, a key metabolic enzyme. Despite its importance for mitochondrial function, very little is known about the catalytic mechanisms underlying FAHD1 enzymatic activities, and the architecture of its ligated active site is currently ill defined. These data are for the mouse protein. The structural basis for the bi-functionally of the human protein, i.e., ODx and acylpyruvate hydrolase (ApH), was recently described. These data allow the comparison of both enzymes in terms of structure and functionality. Further, a monoclonal antibody could be produced that is able to recognize mouse FAHD1, but not the human form, whereas a polyclonal α-hFAHD1 (anti human FAHD1) antibody recognizes both proteins. Epitope mapping in combination with our deposited crystal structures revealed the associated amino acid sequence, different among the proteins.