Relative staining intensity of scFv-6E probed by dot-blot against mutant httex1 at specific time points during fibrillogenesisa.

aStaining intensities were determined by dot-blot analysis of httex1-53Q using scFv-6E as a primary antibody. Briefly, purified GST-httex1-53Q protein was ultracentrifuged to remove preformed particles and digested to initiate httex1-53Q aggregation as described in Materials and Methods. Aliquots of httex1-53Q were deposited onto nitrocellulose 0, 1, 6, 24, and 144 hours after initiating aggregation and probed against scFv-6E by dot-blot. Results from duplicate experiments were averaged and normalized to time 0 values. Higher scFv-6E staining is observed at later time points when httex1-53Q is predominately fibrillar but not earlier time points when monomers and oligomers prevail.