Microbial communities in reed and peat wetlands used for treatment of municipal wastewater. The Effects of cold frozen conditions on nitrogen removal and transformation in wetlands purifying wastewater: insights from combined full- and pilot-scale observations

In wastewater treatment, wetlands are regarded as cost-efficient and sustainable purification systems. Currently, different types of wetland are used for year-round treatment of municipal wastewater (the polishing step after the secondary or tertiary units) in cold climate regions. However, there is a lack of understanding regarding the effect of freezing cold conditions on pollutant removal processes, in particular regarding those linked to nitrogen cycling. This study evaluated the effect of cold winter conditions on contaminant removal, with a focus on nitrogen transformation and removal in a reed-based surface flow (Reed-SF) wetland and a peat-based horizontal subsurface flow (Peat-HSSF) wetland. Year-round full-scale wetland monitoring was complemented with pilot-scale laboratory experiments, which allowed to follow the cold-climate induced effects on contaminant removal and nitrogen processes, along with other water quality, environmental, and microbial parameters. Overall, it was observed that the effect of cold climate conditions on nitrogen removal was dependent on the wetland type in combination with the quality of the inflow water. When treating NH4-N rich wastewater, removal of Ntot, as well as NH4-N, was higher in the Peat-HSSF than in the Reed-SF wetland. Under frozen conditions, NH4-N removal decreased slightly but remained positive in the pilot Peat-HSSF, whereas it declined and even turned into leaching in the pilot Reed-SF wetland. Conditions encountered in the Peat-HSSF wetlands (high abundances of active nitrifying bacteria, high levels of DO and redox potential) supported nitrification, which continued under cold conditions. Whereas in the Reed-SF, a low abundance of active nitrifying bacteria was found, especially in the water column under frozen conditions. DO here was low and decreased in cold conditions with ice cover.