Transcriptomic analysis of BMP4/GREM1 stimulation on primary myoblasts

The capacity of muscle to grow or to regenerate is provided by adult stem cells, called satellite cells. Satellite cells can generate proliferating myoblasts, sensitive to MP signaling. To understand the role of this signaling pathway in adult myogenesis, we analyzed the changes in gene expression following treatment of primary myoblasts with BMP4 agonist or GREM1 antogonist. Here we used microarrays and to clarify BMP4/GREM1 signaling and we identified several pathways and cellular processes affected. We cultured satellite cells-derived primary myoblasts in proliferating conditions (20% FBS), transfered them in 2% Horse Serum and stimulated the cells with recombinant BMP4 or GREM1 proteins. Cells were processed for RNA extraction after 24hours.