Non-Thermal Atmospheric Pressure Plasma Induces Epigenetic Modifications that Activate the Expression of Various Cytokines and Growth Factors in Human Adipose Tissue-Derived Stem Cells

In this study, by analyzing the whole genome expression profiles of NTAPP-activated ASCs with RNA-seq, we investigated the mechanism by which NTAPP increases the proliferation of ASCs in order to deduce the common mechanism underlying the physiological effects of NTAPP. We demonstrated that NTAPP increases the expression of genes associated with the activity of various cytokines and growth factors, and downregulates the genes involved in the intrinsic apoptotic pathway by inducing epigenetic modifications. We also showed that nitric oxide (NO) generated from NTAPP is a main component of epigenetic modification by NTAPP. The physiological mechanism of NTAPP proven in this study supports the development of NTAPP as an efficient tool for regenerative medicine.