Data from: Systematic analysis of complex genetic interactions

To systematically explore complex genetic interactions, we constructed
~200,000 yeast triple mutants and scored negative trigenic interactions.
We selected double-mutant query genes across a broad spectrum of
biological processes, spanning a range of quantitative features of the
global digenic interaction network and tested for a genetic interaction
with a third mutation. Trigenic interactions often occurred among
functionally related genes, and essential genes were hubs on the trigenic
network. Despite their functional enrichment, trigenic interactions tended
to link genes in distant bioprocesses and displayed a weaker magnitude
than digenic interactions. We estimate that the global trigenic
interaction network is ~100 times as large as the global digenic network,
highlighting the potential for complex genetic interactions to affect the
biology of inheritance, including the genotype-to-phenotype relationship.