Exosome-microRNAs sequencing in chickens

Mycoplasma gallisepticum (MG) infects chickens, causes chronic respiratory diseases (CRD) and severely damages the poultry industry. Our previous studies have confirmed that the functional roles of several miRNAs in MG infection of chicken, it is largely unclear how they regulate intercellular communication through exosomes. Although exosomal miRNAs have been demonstrated to produce an important effect on microbial pathogenesis and inflammatory response as crucial regulatory noncoding RNAs, the mechanism by which exosomal miRNAs regulate MG-induced inflammation remains to be elucidated. In this study, the expression of exosome-microRNA derived from MG-infected chicken type pneumocytes was screened by deep sequencing, and the target genes and function of differentially expressed miRNAs were predicted.