HIV DAb-seq of ART-treated individuals

Despite effective antiretroviral therapy (ART), HIV remains an incurable disease. The latent HIV reservoir is a central obstacle to a cure. It consists of rare CD4+ cells harboring replication-competent proviral DNA that inevitably resumes viremia upon ART interruption. Despite their importance, little is known about the properties of these latently infected CD4+ T-cells. In this study, we investigate the immunophenotype of HIV-infected CD4+ T-cells from people living with HIV (PLWH) who have been successfully treated with ART for at least two years. By leveraging and improving our high-throughput single-cell technology, DAb-seq, we detect infected CD4+ T-cells in peripheral blood mononuclear cells (PBMCs) of PLWH using a panel of 10 HIV DNA-targeting amplicons, while simultaneously... (for more see dbGaP study page.)