Regulatory divergence during mouse limb evolution. Regulatory divergence during mouse limb evolution

Genome-wide comparative analyses of the open chromatin profiles between equivalent stages of mouse and pig limb bud development reveal the extensive functional divergence of their limb regulomes. These alterations affect evolutionary conserved regions located in the genomic landscapes of genes with essential functions during limb development. This analysis uncovers the widespread regulatory changes that appear to underlie the morphological diversion of the artiodactyl limb from the pentadactyl blueprint of tetrapods. Overall design: ATAC-seq analysis on dissected E10.5 and E11.5 mouse forelimb buds