Transcriptional profiling of HBV-naïve subjects after vaccination against Hepatitis A/B viruses, Diphtheria/Tetanus toxoids and Cholera.

Mechanisms of poor responses to vaccines remain unknown. Hepatitis B virus-naïve elderly subjects received three vaccines, including a vaccine against hepatitis B virus (HBV). Transcriptomic profilling of blood collected pre-vaccination and post-vaccination was performed in order to identify candidate biomarkers of antibody response to the different vaccines. One hundred and seventy four (174) generally healthy, Hepatitis B virus (HBV) naïve, adult residents of Québec were vaccinated with two doses of Twinrix® (HBsAg and Hepatitis A virus - Glaxo Smith-Kline), generic Tetanus-diphtheria booster (tetanus and diphtheria - Sanofi-Pasteur), and Dukoral (recombinant cholera toxin B subunit and whole killed Vibrios - Sanofi Pasteur) according to the respective product labels. Blood samples were taken seven (7) post vaccination. Blood samples were conserved in PAXgene tubes. RNA was extracted and hybridized to Affymetrix arrays. 2 technical replicates were included in the study. technical replicate: EM131_1009_V3, EM131_1009_V3_rep1 technical replicate: EM131_2036_V3, EM131_2036_V3_rep1