RNA-Seq of UXT KO in Primary Sertoli cells

We created a knockout model of ART-27(UXT) specially in the Sertoli cells of the mouse testes. In order to see how loss of ART-27 effects the Sertoli cell transcriptome we isolated Sertoli cells from WT and KO animals. The WT cells were infected with a vector only construct, and the KO cells were infected with CRE producing construct. Overall design: examination of transcriptional changes in WT primary Sertoli cells and KO Primary Sertoli cells. Each condition was done in triplicate