APOBEC3A nuclear RNA variant analysis

To identify candidate APOBEC3A edit sites on pre-ribosomal and other nuclear RNAs, we profiled nuclear RNAs from MCF10A breast epithelial cells by Illumina sequencing. Treatment conditions were a non-targeting siRNA (siNT) negative control, siAPOBEC3A siRNA pool, and siAPOBEC3A siRNA individual (Dharmacon/Horizon Discovery). Cells were treated with siRNAs for 72 hours. Nuclei were purified and nuclear RNA was harvested using TRIzol. The Yale Center for Genome Analysis performed prepared 150 bp paired end libraries for sequencing without polyA enrichment or ribosomal RNA depletion steps. 2 replicates per treatment.