Engineering Desiccation Tolerance in Escherichia coli
收藏PubMed Central2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC92041/
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资源简介：
Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation over phosphorus pentoxide. The phase transition temperatures and vibration frequencies (P⩵O stretch) in phospholipids suggested that sucrose maintained membrane fluidity during cell dehydration.
提供机构：
American Society for Microbiology (ASM)
