Short- and long-read sequencing of bacteriophages using ultra low starting DNA input

In this study, we wanted to assess the feasibility of sequencing samples with very low DNA starting material and to determine the impact of different DNA input on sequencing quality using both Illumina short-read and Nanopore long-read technologies. Specifically, we analyzed the quantity and quality of output sequences (and results such as genome assemblies) for different ranges of input DNA concentrations starting at the recommended concentration for each manufacturer. We concluded that it is definitely achievable to perform sequencing of high quality with DNA inputs that are much lower than manufacturers recommendations or requirements.