Effects of CLP and CORM-2 on MLC phosphorylation in rat ileum.

The lysates of ileum from different groups of rats were subjected to Western blotting analysis with anti-MLC and phosphorylation-specific antibody for p-MLC. Typical Western blots and Means±SD of the ratios of p-MLC/MLCcalculated based on the densities of bands on Western blots from four groups are presented. (A): Time course of MLC phosphorylation in CLP (C) and CORM-2-treated (T) CLP rats (5 rats each time point per group) aP < 0.05 compared to CLP group. (B) The MLC phosphorylation levels at 24 h of each experimental group. aP < 0.05 compared to Sham rats; bP < 0.05 compared to CLP rats; cP < 0.05 compared to CLP rats treated with CORM-2.