Development of miniBac-seq to profile bacterial transcriptome from ultra-low input RNA amount

We developed miniBac-seq, a strand-specific method for high-quality bacterial RNA-seq library construction from sub-nanogram of total RNA, equivalent to a few thousand bacterial cells; we also tested its potent to be adopted as a single cell RNA-seq method. Here we provide the supporting data to the paper introducing this technology. Overall design: We used E. coli K12 BW25113, typically cultivated in M63B1 broth and extracted total RNA at the Log phase. Fig2 Impact of customized rRNA depletion method on transcript quantification; Fig4 Overall performance of miniBac-seq; Fig 5 Detection limit of miniBac-seq, via library construction from a gradient of input RNA (5 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg); Fig 6 Impact of barcode introduced at RT on transcript quantification