DNase Hypersensitivity Peaks from Rat Schwann Cells. Rattus norvegicus

We utilized DNase-Seq to identify open chromatin regions within Schwann cells. This data was used in conjunction with sequence conservation and transcription factor binding site prediction to identify putative cis-regulatory elements critical for Schwann cells. We used three independent biological replicates, and for our studies used sample 'barcode4' as a representative sample for all three. DNase HSS peaks were identified using F-Seq and HOMER, and overlapped to prioritize candidate regions. These regions were then verified using luciferase assays. Overall design: Performed DNase HSS on three independent biological replicates of S16 rat Schwann cells