Additional file 1 of Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: a valuable tool for population genetic analysis and SIT applications

Additional file 1: Basic characteristics and PCR amplification conditions of 50 A. fraterculus microsatellite loci. Detailed legend: N: number of individuals analyzed; Dye: fluorescent label incorporated in forward primers; T: optimized annealing temperature; [MgCl2]: optimized magnesium chloride concentration; Size: size of PCR product predicted by Primer 3 software. A: Number of alleles detected. NA: no PCR amplification observed; ND: number of alleles not determined; MP: multiple peaks or bands observed in agarose gels or capillary electrophoresis analysis; CP: complex peaks: complex pattern of peaks observed in capillary electrophoresis analysis, difficult allele scoring. * Primers designed by GIS Company using Designer PCR software v 1.03 (Research Genetics, Inc.). (XLS 42 KB)