five

mTEChi and mTEClo RNAseq profiling

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NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE178456
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Increased expression of Padi genes in mTEChi Total RNA isolated by Trizol extraction from MHC-II high or low mTECs (from pooled thymi of B6 mice) was used to generate poly-A-selected transcriptome libraries using the non-directionnal TruSeq V3 RNA Sample Prep Kit. Paired-end sequencing (2x100bp) was performed for mTEChi and single-end sequencing (50bp) for mTEClo. Sequencing was done on the Illumina HiSeq2000 machine.
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2021-09-29
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