Comparison of expression profile between fibroblasts taken from young (23 years old) and aged (70 years old) donors

Several features differentiate aged cells from young cells, many of which are due to changes in gene expression during the aging process. The mechanisms of altered gene expression in aging cells remain incompletely understood, and we hypothesized that long non-coding (lnc) RNAs mediate at least some of these changes. To determine candidate lncRNAs which are associated with aging process, we screened for alterations in lncRNA expression with aging in skin fibroblasts. Twenty-eight lncRNAs were found to be expressed more than 2-fold in aged fibroblasts, and thirty-five lncRNAs expressed less than 0.5-fold. Normal human dermal fibroblasts taken from young (23 years old) and aged (70 years old) donors' abdomen were cultured with 10% feratl bovine serum-containing Dulbecco’s modified Eagle’s medium. Cell lysate from three independent dishes were put into one tube. Total RNA was isolated using RNeasy Mini Kit (QIAGEN), and labeled with Low Input Quick Amp Labeling Kit (Agilent). Following hybridization with Gene Expression Hybridization Kit (Agilent), microarray was scanned by DNA Microarray Scanner (Agilent).