DFI Sexual Abstinence raw data

This record contains raw data related to article "Do the reduction of sexual abstinence influence sperm DNA fragmentation? Exploring the relationship between the two parameters."

Abstract

Background

The exact mechanism behind age-related sperm decline remain unclear. Factors like increased free radicals and prolonged sexual abstinence (SA) have been explored in literature. Recent studies suggest that shorter SA may positively affect sperm parameters, though results are controversial.

Objectives

Our retrospective analysis aimed to investigate whether reduced SA across pantients’ age really benefits sperm DNA fragmentation (SDF) in patients undergoing in vitro fertilization (IVF).

Methods

1030 patients underwent both sperm analysis and SDF evaluation. The dataset was both studied entirely and split in groups with generalized linear model and quantile regression analyses. Primarily, we considered 4 groups based on patients’ age (≤38 and >38 years) and days of SA (0-3 and 4-7 days); then, we examined 2 age-based groups (≤38 and >38 years).

Results

Sexual abstinence (SA) emerged as a significant predictor of sperm DNA fragmentation (DFI) (P=0.020), with a stronger effect observed in patients >38 years (P=0.013). No significant interaction between age and SA was found in the general model (P=0.524), but stratified analysis revealed a threshold effect, where prolonged SA was particularly detrimental in older individuals. Quantile regression further confirmed that SA had the most pronounced impact on patients with higher baseline DFI, suggesting that oxidative stress accumulates disproportionately in this subgroup.

Conclusion

Our findings highlight the complex interplay between age, SA, and DFI, demonstrating that prolonged abstinence is especially detrimental for elder men. Unlike previous studies, we introduce a threshold effect, emphasizing that the impact of SA is not uniform across all individuals. The use of quantile regression allows a more refined assessment, revealing that those with pre-existing sperm DNA damage are more susceptible. These results suggest that standard WHO-recommended abstinence windows may not be optimal for all patients, particularly in ART settings, advocating for more individualized SA guidelines to improve reproductive outcomes.