species_chloroplast_latlong

"species_chloroplast_latlong.csv" contains collection data and chloroplast genotypes for each sample in the hybridization study.
**The "Well" column indicates the plate number and well where the sample was located on the DNA dilution plates.
**The "Site" column contains a three-letter code for the collection site, which corresponds to a collection site description that can be found in the Supplementary Material fo the paper.
**The "Individual" column contains the site code plus number used to uniquely identify each individual.
**The "Initial Species Determination" column lists the species as it was determined either during collection or after preliminary analysis.
**The "Species based on genetic data" column lists the final species determination. Identification of R. vestitus and R. anglocandicans individuals is described in a manuscript that is in revision for the journal Biological Invasions.
**The "ndh Allele" column gives the size of the PCR fragment produced by amplifying the ndhF chloroplast region (using primers and PCR conditions are described in the paper), while the "Peak Height" column gives the height of the peak detected on an ABI3100 capillary sequencer for this allele, and "Run" lists the identity of the run as produced by the data collection software.
**The "trnK allele" column gives the result of a CAPS marker in the trnK region of the chloroplast genome: the "cut" allele has a HindIII site while the "uncut" allele does not. **"pch for R" contains the number of the symbol used to represent each individual in Principal Coordinate Analysis in R, based on the ndhF allele.
**"color for R" contains the colors initially used to represent each individual in Principal Coordinates Analysis in R, based on the trnK allele and whether the individual is a member of the R. fruticosus agg. or not. All "magenta" individuals were later changed to "red" so that the color would only represent the genetic results.
**"LatOrder" contains a number for each population, in order of increasing latitude.
**"Latitude" and "Longitude" contain decimal coordinates where the sample was collected, obtained using a Garmin GPSMAP 60CS in the WGS84 datum.
**"Collection date" contains the date that leaf tissue was collected in the field, in YYYYMMDD format.
**"Comment" contains any additional information, such as whether a pressed voucher was collected, whether the sample was excluded from analysis, or information about morphology or location.