Sphagnum phyllosphere metagenome Targeted loci environmental

Here we explore the impact of experimental warming on the microbial community structure associated with the widespread and ecologically relevant moss species Sphagnum (peat moss). The diversity and composition of Sphagnum associated bacterial communities was determined by applying a high-throughput sequencing-based protocol that targets PCR-generated amplicons from the V4 variable regions of the 16S rRNA gene using the primer set 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3')