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Bulk transcriptomic profiling of dermal macrophage subtypes

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https://www.ncbi.nlm.nih.gov/sra/SRP421312
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Viable mouse dermal macrophage (CD45+F4/80+SiglecF-) subtypes were identified by antigen expression combination of Ly6C, FRb and CD163 and sorted from dorsal skin of 7 week-old C57BL/6 female mice by FACS. Samples were paired within individual mice (5 mice each). RNA from 500 cells were isolated and profile for transcriptome by sequencing. Overall design: Dermal macrophage subtypes were sorted by surface antigens by flow cytometry followed by transcriptomic profiling with bulk RNA-seq.
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2024-12-26
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