Disruption of RNAPII transcription elongation links Oncogenic splicing factor mutations to replciation stress and targetable alterations in chromatin landscape [RNA-seq]

To investigate the impact of altered transcription and chromatin on steady state transcript levels, we peformed gene expression and splicing analysis in K562 cells (wild type and SF3B1K700E mutant) at 4 and 8 days after mutant allele expression We then performed gene expression profiling analysis using data obtained from RNA-seq at two time points. Differential gene expression and splicing analysis for K562 (wild type vs SF3B1K700E mutant) cells