Genome wide screening for binding target of ELF3 transcription factor in biliary epithrial cell line HBDEC2. Genome wide screening for binding target of ELF3 transcription factor in biliary epithrial cell line HBDEC2

ELF3, a member of E26 transformation-specific transcription factors, is inactivated in a range of cancers including biliary tract cancer. We performed chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-Seq) to identify direct ELF3 target genes. The number of uniquely mapped reads identified by ChIP-Seq was 109.9 M for the ELF3 binding site and 59.7 M for the control input DNA. These read data were evaluated with MACS 1.4, and 13,960 peaks were identified throughout the genome with a false discovery rate (FDR) of < 0.1. Overall design: ELF3 ChIP-Seq was performed on ELF3 gene-knockout HBDEC2 cells with ELF3 tet-ON system.