Effects of the Rad53 S/T mutations on DNA checkpoint-mediated function.
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a)Stationary-phase yeast cells were treated with 20 mM HU or 0.02 mM MMS in fresh YPD for 6 h, recover in fresh YPD for 8 h and the fractions of cells with an elongated bud (length of bud 1.5 times that of the mother) were counted.b)Equivalent numbers of yeast cells were treated with 20 mM HU or 0.02 mM MMS for 2 h and then same cell diluted before being spread onto YPD plates for counting colony-forming units from few (+) to many (+++++) after a 2-d incubation at 30°C. Indicates * P valueP valuec)rad53-S4A mean a quadruple mutant of S455A/S457A/S459A/S461A; rad53-S4D mean a a quadruple mutant of S455D/S457D/S459D/S461D.
创建时间：
2015-12-02
