Tissue-based mapping of the fathead minnow (Pimephales promelas) transcriptome and proteome

Omics approaches are broadly used to explore endocrine and toxicity-related pathways and functions. Nevertheless, there is still a significant gap in knowledge in terms of understanding the endocrine system and its numerous connections and intricate feedback loops, especially in non-model organisms. The main purpose of this analysis was to generate tissue-specific omics data in order to support future aquatic ecotoxicogenomic and endocrine-related studies as well as to improve our understanding of the fathead minnow as an ecological model. Overall design: All fish husbandry was conducted under the supervision of the University of Florida Institutional Animal Care and Use Committee. Adult fathead minnows (Pimephales promelas) were obtained from an in-house culture at the Aquatic Toxicology Core Laboratory at the University. The main objective of this study was to increase the value of the FHM as a model by creating comprehensive transcriptomic and proteomic databases. This study also aims to survey tissue-specific baseline transcriptomic and proteomic expression profiles in adult male FHM to support aquatic ecotoxicogenomic studies.