Comprehensive analysis of microRNA after moderate intensity exercise in mice. Comprehensive analysis of microRNA after moderate intensity exercise in mice

Moderate exercise is important for health; however, individuals differ in moderate intensity and it is difficult to identify. The purpose of this study was to identify new objective indicators to determine effective exercise intensity. Overall design: The mice were randomly divided into the three groups: normal caged mouse group (control group, n = 3), aerobic exercise group (AE group, n = 3), and resistance exercise group (RE group, n = 3). The mice in AE group and RE group were exercised in each effective intensity and blood samples were obtained. Total RNA was isolated and analyzed using 3D-Gene miRNA Labeling kit and 3D-gene Human miRNA Oligo Chip (Toray Industries, Inc., Kanagawa, Japan).