A Comparative Investigation of Catecholamines and Glucocorticoids Impact on Glioblastoma Invasive Behavior via 2D and 3D Cell Culture

This dataset comes from the study “A Comparative Investigation of Catecholamines and Glucocorticoids Impact on Glioblastoma Invasive Behavior via 2D and 3D Cell Culture.”It includes data from experiments using U87-MG glioblastoma cells treated with epinephrine (200 nM, 2 μM) and hydrocortisone (500 nM, 5 μM) for four days. The goal was to examine how stress hormones influence cell invasion, stiffness, and epithelial-to-mesenchymal transition (EMT) markers.

The dataset contains Bio-AFM data (raw .nid files and extracted parameters), flow cytometry data (.fcs files), and microscopy data (raw images and quantified measurements).

All experiments were performed on U87-MG cells under identical culture conditions. The results show that epinephrine increased glioblastoma invasiveness by reducing cell stiffness and elevating vimentin levels, while hydrocortisone made cells stiffer and less migratory, despite a similar rise in vimentin expression.