Variants_AeFv_and_GEnetic_Distance.xlsx

The sequences corresponds to 165 nucleotides region of the NS5 gene. Many sequences were identical to others and therefore considered to represent the same "variant". There were 28 variants of AeFv. The DENV-1 sequence obtained in this study AeFV_467yuc16, AeFV_464yuc16 (Variant 1) and AeFV_326yuc16 (Variant 2).List of sequences were aligned and edited using Mega v.7.
The similarity and identity of the sequences were calculated using the MatGat v.2 software. Genetic distances among variants of AeFlav from Ae. aegypti were calculated using the Mega v.7 software24 with 10,000 random permutations. The DnaSP v.5.10 software was also used to analyze genetic variants of sequences downloaded from GenBank for phylogenetic analysis.