Effects of CLP and CORM-2 on the expression levels of tight junction proteins.

Ileum from different groups of rats were collected and homogenized. The tissue lysates were subjected to Western blotting analysis using specific antibodies for ZO-1, claudin-1 or occludin with β-actin as an endogenous control. Typical Western blots are presented (A). The band intensity was quantified using densitometry. The means±SDs of ZO-1/β-actin ratios (B); claudin-1/β-actin ratios (C); and occludin/β-actin ratios (D) from different groups of rats are presented. aP < 0.05 compared to Sham rats; bP < 0.05 compared to CLP rats; cP < 0.05 compared to CLP rats treated with CORM-2.