Single-Cell Transcriptome of Tumor-educated Lung epithelial cells

We performed single-cell RNA sequencing on epithelial cells, isolated from lungs of normal and tumor-bearing mice, to shed light on the function and phenotype changes of lung epithelial cells in pre-metastatic niche. Lung tissues from tumor-bearing mouse and normal mouse (as a control) were digested into single-cell suspensions and CD45- SftpC+ epithelial cells were purified by FACS, followed by 10× library preparation and sequencing. All procedures were performed according to the standard manufacturer’s protocol (CG000206 RevD). Sequencing libraries were sequenced on NovaSeq6000 (Illumina) using 2x150 chemistry and quantified using a High Sensitivity DNA Chip (Agilent) on a Bioanalyzer 2100 and the Qubit High Sensitivity DNA Assay (Thermo Fisher Scientific). Single-cell RNA-seq data processing Reads were processed using the Cell Ranger 2.1.0 pipeline with default and recommended parameters.