Study on the Effect of Modified Liu-an Decoction on Th1/Th2 Function in Guinea Pigs with Cough Variant Asthma through the Notch Signal Pathway

60 SPF Hartley guinea pigs were randomized into six groups (n=10): the blank control group, model group, montelukast sodium group (MS group), as well as MLAD group of low-dose, mid-dose and high-dose. Intraperitoneal injection of ovalbumin, aluminum hydroxide sensitization, and nebulized inhalation of ovalbumin were performed in all guinea pigs of non-blank control groupto induce CVA. Cough frequency and airway resistance were recorded. The chronic inflammatory infiltration in the airways was detected by using HE and Masson staining smears. The IL-2, IL-4, IL-12, and IL-13 levels were measured by enzyme-linked immunoassay (ELISA). The expression of Delta, Jagged1, Notch1, and NICD proteins in the lung tissues were analyzed by Western blot.